6LKL

Two-component system protein mediate signal transduction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	71.375, 85.932, 71.515
Unit cell angles	90.00, 96.74, 90.00

Refinement procedure

Resolution	45.358 - 2.213
R-factor	0.172
Rwork	0.171
R-free	0.20010
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.730
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.210	2.210
Rmerge	0.113	0.411
Rmeas	0.119	0.488
Rpim	0.063	0.259
Number of reflections	41995	4135
<I/σ(I)>	9.1
Completeness [%]	98.0	97.2
Redundancy	3.5	3.5
CC(1/2)	0.991	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	60% Tascimate pH 7.0