6LKK

Two-component system protein mediate signal transduction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	52.150, 67.444, 87.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.307 - 1.502
R-factor	0.1757
Rwork	0.175
R-free	0.19580
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.560
High resolution limit [Å]	1.500	1.500
Rmerge	0.065	0.451
Rmeas	0.074	0.497
Rpim	0.021	0.135
Number of reflections	47810	3920
<I/σ(I)>	15.36	2.42
Completeness [%]	99.9	100
Redundancy	7.1	6.7
CC(1/2)	0.997	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M Tris pH 7.5-7.9, 21% PEG 3350