6LKI

Two-component system protein mediate signal transduction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	95.231, 106.642, 120.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.749 - 1.781
R-factor	0.2104
Rwork	0.209
R-free	0.23620
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.486
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.780	1.780
Rmerge	0.177	1.274
Rmeas	0.185	1.345
Rpim	0.053	0.419
Number of reflections	57600	4922
<I/σ(I)>	15.83	1.79
Completeness [%]	98.1	85.2
Redundancy	12.2	8.8
CC(1/2)	0.991	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	60% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0