6LKH
Two-component system protein mediate signal transduction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.672, 64.118, 106.832 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 48.661 - 2.534 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25890 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.617 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.534 | 2.550 |
| Rmerge | 0.179 | 1.180 |
| Rmeas | 0.194 | 1.286 |
| Rpim | 0.074 | 0.505 |
| Number of reflections | 42764 | 3775 |
| <I/σ(I)> | 13.396 | 2.766 |
| Completeness [%] | 99.0 | 99.4 |
| Redundancy | 6.9 | 6.3 |
| CC(1/2) | 0.966 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.2 M Ammonium citrate tribasic pH 7.0, 20% PEG 3350 |
