6LKB

Crystal Structure of the peptidylprolyl isomerase domain of Arabidopsis thaliana CYP71.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	277
Detector technology	CCD
Collection date	2018-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	60.954, 60.928, 127.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.477 - 1.651
R-factor	0.1899
Rwork	0.189
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a2n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.480	1.690
High resolution limit [Å]	1.650	1.650
Number of reflections	57328	3778
<I/σ(I)>	5
Completeness [%]	99.0
Redundancy	4
CC(1/2)	0.850	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.1M MES (pH 6.5), 1.5M ammonium sulphate, 0.02M cobalt chloride hexahydrate