6LK7
crystal structure of Os1348 from Pseudomonas sp. Os17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-04-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.607, 33.610, 66.217 |
| Unit cell angles | 90.00, 92.76, 90.00 |
Refinement procedure
| Resolution | 44.961 - 1.903 |
| Rwork | 0.170 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ideal helix |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.549 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMPLE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.172 | 0.700 |
| Number of reflections | 20568 | 2065 |
| <I/σ(I)> | 14.3 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% (w/v) polyethylene glycol monomethylester 2000, 0.2 M MgCl2 |
