6LK6
MLKL mutant - T357AS358A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.821, 75.709, 128.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.320 - 2.410 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m67 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.370 |
| High resolution limit [Å] | 2.320 | 5.970 | 2.320 |
| Rmerge | 0.163 | 0.136 | 0.128 |
| Rmeas | 0.180 | 0.150 | 0.157 |
| Rpim | 0.074 | 0.062 | 0.091 |
| Total number of observations | 73178 | ||
| Number of reflections | 13419 | 980 | 23 |
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 75.3 | 99.9 | 2.6 |
| Redundancy | 5.5 | 5.7 | 1.1 |
| CC(1/2) | 0.971 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 14%(w/v) PEG 6000, 5% EG, 0.1M HEPES(pH7.5) |
