6LK6

MLKL mutant - T357AS358A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99
Spacegroup name	C 2 2 21
Unit cell lengths	70.821, 75.709, 128.494
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.320 - 2.410
R-factor	0.2111
Rwork	0.209
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m67
RMSD bond length	0.005
RMSD bond angle	1.247
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.370
High resolution limit [Å]	2.320	5.970	2.320
Rmerge	0.163	0.136	0.128
Rmeas	0.180	0.150	0.157
Rpim	0.074	0.062	0.091
Total number of observations	73178
Number of reflections	13419	980	23
<I/σ(I)>	5.9
Completeness [%]	75.3	99.9	2.6
Redundancy	5.5	5.7	1.1
CC(1/2)		0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14%(w/v) PEG 6000, 5% EG, 0.1M HEPES(pH7.5)