6LJY
A Crystal Structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.137, 54.560, 66.095 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 19.860 - 1.500 |
| R-factor | 0.1719 |
| Rwork | 0.170 |
| R-free | 0.21090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.826 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.042 | 0.138 |
| Number of reflections | 37055 | 1801 |
| <I/σ(I)> | 22 | 8.2 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 282 | 38%PEG400, 0.1M Tris-HCl, 16.1 mg/mL |
