6LJO
African swine fever virus dUTPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | I 21 3 |
Unit cell lengths | 98.014, 98.014, 98.014 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.060 - 2.280 |
R-factor | 0.2307 |
Rwork | 0.228 |
R-free | 0.27350 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.577 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.073 | 0.534 |
Rmeas | 0.087 | 0.695 |
Rpim | 0.014 | 0.682 |
Number of reflections | 7330 | 371 |
<I/σ(I)> | 1.74 | 2.14 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.87 | 3.69 |
CC(1/2) | 0.997 | 0.777 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.5M NaH2PO4/K2HPO4,PH 6.9 |