6LJB

Crystal Structure of ASFV pS273R protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0083
Spacegroup name	C 1 2 1
Unit cell lengths	220.840, 37.988, 33.026
Unit cell angles	90.00, 93.44, 90.00

Refinement procedure

Resolution	36.740 - 2.487
R-factor	0.2245
Rwork	0.223
R-free	0.25180
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.487	2.500
Number of reflections	9380	418
<I/σ(I)>	11.68
Completeness [%]	95.2
Redundancy	5.5
CC(1/2)	0.993	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.6 mM CoA, 0.2 M Ammonium acetate, 0.1 M Tris, pH8.5, 20% PEG3350.