6LIX

CRL Protein of Arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9779
Spacegroup name	P 1 21 1
Unit cell lengths	38.403, 73.488, 67.712
Unit cell angles	90.00, 98.81, 90.00

Refinement procedure

Resolution	49.476 - 2.385
R-factor	0.224
Rwork	0.223
R-free	0.24690
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.420
High resolution limit [Å]	2.380	6.460	2.380
Rmerge	0.077	0.052	0.268
Rmeas	0.083	0.057	0.292
Rpim	0.032	0.022	0.116
Total number of observations	100253
Number of reflections	14787	772	688
<I/σ(I)>	6.5
Completeness [%]	99.5	99.7	93.4
Redundancy	6.8	6.6	6
CC(1/2)		0.998	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.1 M MES pH 6.5, 12% PEG 20000