6LIG

Crystal structure of human Glutamate oxaloacetate transaminase 1 (GOT1) in complex with AH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	95
Detector technology	CCD
Collection date	2019-04-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	64.870, 90.420, 74.050
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	64.835 - 2.620
R-factor	0.2169
Rwork	0.213
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dnd
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.855	2.714
High resolution limit [Å]	2.620	2.620
Number of reflections	25267	2557
<I/σ(I)>	9.34
Completeness [%]	97.7
Redundancy	2
CC(1/2)	0.989	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Sodium chloride, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350