6LH7
Crystal Structure of Vibrio cholerae Methionine Aminopeptidase with Partially Occupied Metals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9536 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.702, 50.293, 131.684 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 48.226 - 1.473 |
| Rwork | 0.152 |
| R-free | 0.17650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k26 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.883 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.310 | 49.310 | 1.500 |
| High resolution limit [Å] | 1.470 | 8.070 | 1.470 |
| Rmerge | 0.062 | 0.029 | 0.676 |
| Rmeas | 0.067 | 0.032 | 0.739 |
| Rpim | 0.026 | 0.013 | 0.294 |
| Total number of observations | 684923 | 4032 | 31658 |
| Number of reflections | 108944 | 716 | 5255 |
| <I/σ(I)> | 17.6 | 46.6 | 2.7 |
| Completeness [%] | 99.7 | 98.7 | 97.7 |
| Redundancy | 6.3 | 5.6 | 6 |
| CC(1/2) | 0.999 | 0.999 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1 M Hepes pH-7.3 , 10% PEG 3350, 5% Glycerol |
