6LGX
Structure of Rabies virus glycoprotein at basic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.440, 138.447, 61.442 |
| Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
| Resolution | 47.398 - 3.097 |
| R-factor | 0.2442 |
| Rwork | 0.242 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lgw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.739 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.097 | 3.097 |
| Number of reflections | 28680 | 2852 |
| <I/σ(I)> | 14.45 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.8 | 6.7 |
| CC(1/2) | 0.985 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1 M BICINE, 14% Polyethylene glycol 300 |
