6LGW

Structure of Rabies virus glycoprotein in complex with neutralizing antibody 523-11 at acidic pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97890
Spacegroup name	P 21 21 21
Unit cell lengths	82.892, 93.943, 213.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.978 - 2.904
R-factor	0.2202
Rwork	0.218
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m43
RMSD bond length	0.004
RMSD bond angle	0.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	37561	3681
<I/σ(I)>	14.76	0.96
Completeness [%]	100.0	100
Redundancy	10.9	11.3
CC(1/2)	0.982	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	30 mM Diethylene glycol, 30 mM Triethylene glycol, 30 mM Tetraethylene glycol, 30 mM Pentaethylene glycol, 55.5 mM MES monohydrate, 44.5 mM Imidazole, 20% v/v Ethylene glycol, 10% w/v PEG 8000