6LGQ
The crystal complex structure of histidine kinase and response regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.799, 63.068, 85.278 |
| Unit cell angles | 90.00, 102.53, 90.00 |
Refinement procedure
| Resolution | 38.830 - 3.000 |
| R-factor | 0.1881 |
| Rwork | 0.183 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kny |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.026 | |
| Number of reflections | 10464 | 455 |
| <I/σ(I)> | 28.84 | |
| Completeness [%] | 92.2 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M HEPES pH 7.0, 5% PEG6000 |
