6LFS

Poa1p H23A mutant in complex with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-23
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 64
Unit cell lengths	82.429, 82.429, 52.588
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.439 - 1.870
R-factor	0.1802
Rwork	0.176
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lfq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.439	1.930
High resolution limit [Å]	1.870	1.870
Number of reflections	16602
<I/σ(I)>	24.2
Completeness [%]	99.8
Redundancy	5.5
CC(1/2)	0.981	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	Sodium chloride, CAPS, Sodium hydroxide, PEG 8000, Glycerol