6LFQ

Crystal structure of Poa1p in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-23
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	49.195, 59.612, 100.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.806 - 2.359
R-factor	0.1694
Rwork	0.160
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.806	2.444
High resolution limit [Å]	2.359	2.359
Number of reflections	6209
<I/σ(I)>	14
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.971	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	HEPES sodium, Lithium sulfate monohydrate, Glycerol