6LEY

Structure of Sil1G bound FEM1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	95.497, 95.973, 147.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.440 - 2.390
R-factor	0.1992
Rwork	0.198
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	147.480	2.450
High resolution limit [Å]	2.390	2.390
Number of reflections	54296	5321
<I/σ(I)>	12.9
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.0M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic pH 6.9