6LER
169 bp nucleosome harboring non-identical cohesive DNA termini.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98.15 |
| Detector technology | PIXEL |
| Collection date | 2019-08-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 107.338, 116.545, 117.900 |
| Unit cell angles | 61.50, 82.77, 64.23 |
Refinement procedure
| Resolution | 48.120 - 3.000 |
| R-factor | 0.2094 |
| Rwork | 0.208 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ut9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.152 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.056 | 0.883 |
| Rmeas | 0.079 | |
| Rpim | 0.056 | 0.883 |
| Number of reflections | 88754 | 4306 |
| <I/σ(I)> | 12 | 1 |
| Completeness [%] | 98.6 | 93.7 |
| Redundancy | 3.5 | 3.1 |
| CC(1/2) | 0.997 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291.15 | Calcium chloride, potassium chloride, sodium acetate |
