6LE9

The Human Telomeric Nucleosome Displays Distinct Structural and Dynamic Properties

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	106.455, 109.388, 176.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.600
R-factor	0.2402
Rwork	0.238
R-free	0.28050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lz0
RMSD bond length	0.007
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.961	2.899
High resolution limit [Å]	2.518	2.518
Rmerge	0.114	1.506
Rmeas	0.119
Rpim	0.033	0.426
Number of reflections	33289	1665
<I/σ(I)>	14.3
Completeness [%]	94.5	79.6
Redundancy	13	13.4
CC(1/2)	0.998	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Manganase chloride, potassium chloride, potassium cacodylate, MPD and trehalose