6LE6

Structure of LNLPTQGRAR bound FEM1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	94.515, 96.334, 146.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.421 - 2.330
R-factor	0.2059
Rwork	0.204
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.520	2.390
High resolution limit [Å]	2.330	2.330
Number of reflections	57097	5517
<I/σ(I)>	17.9
Completeness [%]	99.3
Redundancy	13.4
CC(1/2)	0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.6M Lithium sulfate, 0.1M BIS-TRIS propane Ph 6.4