6LDV
Structure antibody F9 in complex with methylated peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-08-05 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 49.764, 63.526, 125.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.320 - 1.900 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m63 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.529 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.320 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.193 | 1.213 |
| Rpim | 0.052 | 0.379 |
| Number of reflections | 31867 | 1856 |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 99.2 | |
| Redundancy | 14.4 | |
| CC(1/2) | 0.997 | 0.677 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M potassium iodide 20% PEG3350 |
