6LDU

Crystal structure of CeCht1, a nematode I family chitinase from C. elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	P 1 21 1
Unit cell lengths	47.571, 66.935, 57.352
Unit cell angles	90.00, 102.71, 90.00

Refinement procedure

Resolution	42.927 - 1.701
R-factor	0.168286066436
Rwork	0.166
R-free	0.20885
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w4r
RMSD bond length	0.007
RMSD bond angle	0.906
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.083	0.401
Number of reflections	38049	3724
<I/σ(I)>	9.199
Completeness [%]	99.0
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3.350, Tris