6LDQ

Sucrose-phosphate synthase (tll1590)_27_220_406_426_from Thermosynechococcus elongatus (twinned)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	50.022, 134.059, 60.794
Unit cell angles	90.00, 90.08, 90.00

Refinement procedure

Resolution	19.398 - 1.920
R-factor	0.2422
Rwork	0.240
R-free	0.29080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kih
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.400	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.118	0.625
Number of reflections	60298	4059
<I/σ(I)>	6.7
Completeness [%]	99.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Tris