6LDO

Crystal structure of cystathionine gamma-lyase from Lactobacillus plantarum complexed with L-serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	217.412, 201.149, 113.938
Unit cell angles	90.00, 117.39, 90.00

Refinement procedure

Resolution	35.682 - 2.750
R-factor	0.1632
Rwork	0.161
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4L0O
Data reduction software	HKL-2000 (v1.0)
Data scaling software	SCALEPACK (v1.0)
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.850
High resolution limit [Å]	2.743	5.920	2.750
Rmerge	0.109	0.047	0.436
Rmeas	0.127	0.055	0.508
Rpim	0.065	0.028	0.260
Number of reflections	112736	11352	11268
<I/σ(I)>	6.7
Completeness [%]	99.9	99.4	100
Redundancy	3.8	3.7	3.8
CC(1/2)		0.997	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	KH2PO4, NaH2PO4