6LDG
Crystal structure of the Zn-directed tetramer of the engineered cyt cb 562 variant, C96I AB5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.987 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 53.723, 53.723, 250.757 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.380 - 1.980 |
R-factor | 0.1706 |
Rwork | 0.168 |
R-free | 0.22130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iq6 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.709 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.380 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 15931 | 760 |
<I/σ(I)> | 105.8 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 40 | 40.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 25% PEG 3350, 100 mM HEPES pH 7.5, 200 mM sodium chloride |