6LDE
Crystal structure of the Zn-directed tetramer of the engineered cyt cb 562 variant, C96V AB5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 53.757, 53.757, 250.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.550 - 2.000 |
| R-factor | 0.1818 |
| Rwork | 0.179 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iq5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.050 |
| Number of reflections | 15537 | 1425 |
| <I/σ(I)> | 24.22 | |
| Completeness [%] | 100.0 | |
| Redundancy | 30.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 36% Polypropylene glycol (P400), 100 mM bis-tris, 200 mM magnessium chloride |
