6LD9

Crystal structure of cystathionine gamma synthase from Xanthomonas oryzae pv. oryzae in complex with cystathionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 41
Unit cell lengths	161.146, 161.146, 245.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.060 - 2.500
R-factor	0.1543
Rwork	0.153
R-free	0.18610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ld7
RMSD bond length	0.018
RMSD bond angle	2.204
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.160	0.520
Number of reflections	106893	10462
<I/σ(I)>	25.8
Completeness [%]	99.8
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	0.12 M Ethylene Glycols Mix, 0.1 M Na HEPES/MOPS pH 7.5, 10%(w/v) PEG 4000, 20%(v/v) glycerol