6LCJ
TtGalA, alpha-galactosidase from Thermus thermopilus in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.435, 163.496, 118.650 |
| Unit cell angles | 90.00, 119.92, 90.00 |
Refinement procedure
| Resolution | 34.280 - 2.500 |
| R-factor | 0.1651 |
| Rwork | 0.165 |
| R-free | 0.20170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zy9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.917 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.280 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.081 | 0.490 |
| Number of reflections | 133881 | 13343 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1M sodium acetate pH 4.4, 0.02M CaCl2, 30% MPD |
