6LCH
Pseudomonas aeruginosa 5086 (PA5086)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-10 |
| Detector | RAYONIX SX-165mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.100, 63.200, 66.900 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 32.888 - 1.899 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jkp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.745 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.899 | 1.900 |
| Number of reflections | 18536 | 1291 |
| <I/σ(I)> | 25 | |
| Completeness [%] | 96.6 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.999 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Sodium sulfate decahydrate, 20% (w/v) PEG 3350 |
