6LBT

Crystal structure of yeast Cdc13 and Stn1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	74.971, 84.577, 165.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.207 - 2.600
R-factor	0.2185
Rwork	0.216
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbr 6lbs
Data scaling software	HKL-3000
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.119	0.073	0.863
Rmeas	0.124	0.077	0.899
Rpim	0.035	0.023	0.249
Total number of observations	428976
Number of reflections	33308	3558	3271
<I/σ(I)>	4.1
Completeness [%]	100.0	99.9	100
Redundancy	12.9	11.9	12.7
CC(1/2)		0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	0.225 M ammonium citrate tribasic, 12% PEG3350