6LBK

Structure of rat GLD-2 (Terminal nucleotidyltransferase 2, TENT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.977760
Spacegroup name	P 1 21 1
Unit cell lengths	79.960, 40.720, 105.000
Unit cell angles	90.00, 100.20, 90.00

Refinement procedure

Resolution	47.198 - 2.496
R-factor	0.206856945188
Rwork	0.205
R-free	0.24253
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jnb
RMSD bond length	0.010
RMSD bond angle	1.213
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MrBUMP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.200	2.580
High resolution limit [Å]	2.490	2.496
Rmerge	0.050	0.524
Number of reflections	44966	23510
<I/σ(I)>	21.5
Completeness [%]	99.1
Redundancy	6.5
CC(1/2)	0.999	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	22.4% PEG 3350, 100 mM Tris-HCl pH 8.0