6LBF
Crystal structure of FEM1B
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 127.810, 127.810, 143.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.678 - 3.252 |
R-factor | 0.2216 |
Rwork | 0.218 |
R-free | 0.25250 |
Structure solution method | MAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.370 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 1.980 | |
Number of reflections | 19211 | 1893 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 100.0 | |
Redundancy | 25.2 | |
CC(1/2) | 1.000 | 0.606 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Tris-HCl, pH 8.5, 0.1M Guanidine hydrochloride, 0.8M Potassium sodium tartrate tetrahydrate, 0.5% w/v Polyethylene glycol monomethyl ether 5000 |