6LBE

Crystal structure of bony fish MHC class I binding beta2M-2 for 2.6 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97892
Spacegroup name	P 1
Unit cell lengths	53.190, 65.270, 69.710
Unit cell angles	64.94, 75.67, 73.61

Refinement procedure

Resolution	62.459 - 2.600
R-factor	0.1731
Rwork	0.169
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y91
RMSD bond length	0.008
RMSD bond angle	0.933
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.459	2.030
High resolution limit [Å]	2.030	2.030
Rmerge	0.080
Rpim	0.013
Number of reflections	20364	20364
<I/σ(I)>	17.177
Completeness [%]	82.1
Redundancy	4.2
CC(1/2)		0.080

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 25% Polyethylene glycol 3350