6LBD

shrimp ferritin T158R G159R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 4 3 2
Unit cell lengths	118.781, 118.781, 118.781
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.756 - 1.386
R-factor	0.1384
Rwork	0.138
R-free	0.14800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
Data scaling software	HKL-2000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.400
High resolution limit [Å]	1.350	2.800	1.350
Rmerge	0.125	0.045	1.118
Rmeas	0.126	0.045	1.136
Rpim	0.020	0.007	0.201
Total number of observations	2197255
Number of reflections	58415	7531	1679
<I/σ(I)>	3.1
Completeness [%]	82.7	100	24.3
Redundancy	37.6	36.7	30.9
CC(1/2)		1.000	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Bis-Tris pH 5.5-6.5, 2.0-3.0 M Sodium chloride