6LB5
Crystal structure of dimeric RXR-LBD complexed with full agonist NEt-3IB and TIF2 co-activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.411, 64.026, 46.631 |
| Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
| Resolution | 46.580 - 2.400 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lb4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 20449 | 3277 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.26 | 3.33 |
| CC(1/2) | 0.996 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Imidazole 20 % PEG 6000 |
