6LB3
Crystal structure of PA4674 in complex with its operator DNA (18bp) from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.284, 95.570, 128.857 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 47.201 - 2.497 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3trb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.497 | 2.500 |
| Rmerge | 0.105 | 0.931 |
| Number of reflections | 47794 | 2352 |
| <I/σ(I)> | 20.1 | 1.64 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1M MES, 35% MPD, 0.2M lithium sulfate |
