6LAU
the wildtype SAM-VI riboswitch bound to SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-07-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.102 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.088, 85.021, 93.392 |
| Unit cell angles | 90.00, 105.78, 90.00 |
Refinement procedure
| Resolution | 39.729 - 3.109 |
| R-factor | 0.1937 |
| Rwork | 0.191 |
| R-free | 0.25160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6las |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.390 | 3.100 |
| Rmerge | 0.148 | 0.040 | 1.125 |
| Rmeas | 0.161 | 0.043 | 1.232 |
| Rpim | 0.061 | 0.016 | 0.492 |
| Total number of observations | 100278 | ||
| Number of reflections | 14922 | 752 | 676 |
| <I/σ(I)> | 2.7 | ||
| Completeness [%] | 97.8 | 94.6 | 91.5 |
| Redundancy | 6.7 | 6.6 | 6 |
| CC(1/2) | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M sodium acetate trihydrate pH 4.6, 10% w/v polyethylene glycol 4,000 |
