6LAD

Crystal structure of Amuc_1100 from Akkermansia muciniphila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	116.657, 123.560, 131.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.933 - 2.700
R-factor	0.215
Rwork	0.213
R-free	0.24900
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.063	0.032	0.856
Rmeas	0.067	0.034	0.901
Rpim	0.021	0.011	0.278
Total number of observations	523303
Number of reflections	51987	5446	5157
<I/σ(I)>	11.7
Completeness [%]	98.6	98.1	99.6
Redundancy	10.1	9.7	10.2
CC(1/2)		0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.1 M Tris-HCl, pH 8.0, 40% PEG 1000