6LA2

343 bp di-nucleosome harboring cohesive DNA termini assembled with linker histone H1.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2018-09-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	107.755, 205.904, 237.677
Unit cell angles	90.00, 97.19, 90.00

Refinement procedure

Resolution	49.350 - 3.890
R-factor	0.2005
Rwork	0.199
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lab 4qlc
RMSD bond length	0.005
RMSD bond angle	1.207
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	4.100
High resolution limit [Å]	3.890	3.890
Rmerge	0.071	1.747
Rmeas	0.084
Rpim	0.044
Number of reflections	93763	88289
<I/σ(I)>	11.4
Completeness [%]	99.3	96.1
Redundancy	6.9	6.7
CC(1/2)	1.000	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291.15	Calcium chloride, Potassium chloride, Sodium acetate