6L9U

Crystal structure of mouse TIFA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-06
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	I 2 2 2
Unit cell lengths	55.527, 79.007, 93.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.429 - 2.601
R-factor	0.2268
Rwork	0.224
R-free	0.26830
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.430	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.044	0.636
Number of reflections	6561	331
<I/σ(I)>	48.9
Completeness [%]	99.5
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	MES pH 6.4, MPD