6L9K
H2-Ld a1a2 complexed with A5 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 76.730, 76.730, 73.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.263 - 1.800 |
| R-factor | 0.1975 |
| Rwork | 0.194 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tjh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.300 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.036 | 0.089 |
| Number of reflections | 23063 | 2310 |
| <I/σ(I)> | 12.3 | 4.8 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 1.1 M ammonium tartrate dibasic pH 7.0 |
