6L9I

Crystal Structure of Lactobacillus farciminis Oxalate Decarboxylase Formate Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	92.957, 122.842, 115.223
Unit cell angles	90.00, 92.47, 90.00

Refinement procedure

Resolution	48.000 - 2.790
R-factor	0.1986
Rwork	0.197
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uw8
RMSD bond length	0.007
RMSD bond angle	1.109
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.920
High resolution limit [Å]	2.790	2.790
Rmeas		0.751
Number of reflections	62238	5989
<I/σ(I)>	8.25	2.857
Completeness [%]	98.8
Redundancy	6.3
CC(1/2)	0.952	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	18% PEG 3350, 0.2M NH4Cl, 0.2M Sodium dihydrogen phosphate, 0.2M Bis-Tris pH5.5