6L9F

Crystal structure of TEAD4 in complex with a novel FAM181A peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00928
Spacegroup name	P 64 2 2
Unit cell lengths	110.009, 110.009, 182.711
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.094 - 2.555
R-factor	0.1959
Rwork	0.190
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jua
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.550	6.920	2.550
Rmerge	0.185	0.052	0.901
Rmeas	0.191	0.054	0.933
Rpim	0.050	0.015	0.239
Total number of observations	320535
Number of reflections	21907	1233	1064
<I/σ(I)>	6.3
Completeness [%]	99.8	97.8	100
Redundancy	14.6	13	15
CC(1/2)		0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6