6L94
The structure of the dioxygenase ABH1 from mouse
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 103.202, 103.202, 86.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.178 - 3.100 |
| R-factor | 0.279383755835 |
| Rwork | 0.278 |
| R-free | 0.29689 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xeg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.660 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.180 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.188 | 0.800 |
| Number of reflections | 8262 | 1216 |
| <I/σ(I)> | 7.6 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.986 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 15-19% PEG 3350, 0.1 M Na-citrate pH 5.5, 0.1 M NaCl |
