6L8O
Crystal structure of the K. lactis Rad5 (Hg-derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 187.635, 187.635, 197.544 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.909 - 3.300 |
| R-factor | 0.1937 |
| Rwork | 0.191 |
| R-free | 0.22940 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.496 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.132 | 3.566 |
| Number of reflections | 31355 | 1540 |
| <I/σ(I)> | 39.8 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 56.1 | 50.7 |
| CC(1/2) | 1.000 | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1M HEPES pH 7.5, 0.9M Ammonium citrate tribasic pH 7.0 |
