6L86
The structure of SfaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 150.173, 150.173, 387.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.100 - 2.230 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dch |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.558 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Number of reflections | 82024 | 1390 |
| <I/σ(I)> | 30.556 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.6 | |
| CC(1/2) | 0.704 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 2.1M D/L-malic pH 7.0 |
