6L81

Crystal structure of Homo sapiens GCP5 N-terminus and Mozart1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9998
Spacegroup name	P 21 21 2
Unit cell lengths	88.390, 96.144, 51.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.170 - 2.197
R-factor	0.222847826508
Rwork	0.219
R-free	0.26139
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.841
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.200	2.643
High resolution limit [Å]	2.190	2.197
Number of reflections	76023	13594
<I/σ(I)>	30.9
Completeness [%]	99.9
Redundancy	5.1
CC(1/2)	0.997	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium iodide, 20% PEG 3350