6L6B
X-ray structure of human galectin-10 in complex with L-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-10-05 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 48.820, 48.820, 261.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.546 - 1.802 |
| Rwork | 0.213 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qkq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.550 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 18129 | 1223 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 99.5 | |
| Redundancy | 18.6 | |
| CC(1/2) | 0.997 | 0.977 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.6 M ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10 % (v/v) 1,4-dioxane |
